Isozymes of α-Amylase Induced by Gibberellic Acid in Embryo-less Grains of Barley
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منابع مشابه
Isozymes of alpha-Amylase Induced by Gibberellic Acid in Embryo-less Grains of Barley.
Yomo (8) and Paleg (5) independently fouind that the induction of a-amylase in the embryo-less grain was stimulated by gibberellin. This fact was confirmed by several investigators (1, 4, 7). Recently one of the authors and his co-workers found that helminthosporol and its air-oxidation derivative, helminthosporic acid, had the same effect as gibberellin. We have investigated isozyme(s) of a-am...
متن کاملalpha-Amylase Isozymes in Gibberellic Acid-treated Barley Half-seeds.
The presence of multiple forms of alpha-amylase in gibberellic acid-treated embryoless barley half-seeds was demonstrated by separation on diethylaminoethyl-Sephadex and isoelectric focusing polyacrylamide gel disc electrophoresis. Two major alpha-amylase fractions (A and B), each consisting of two to three isozyme components, were purified. alpha-Amylase fractions A and B were distinguishable ...
متن کاملMultiple forms of amylase induced by gibberellic acid in isolated barley aleurone layers.
The addition of gibberellic acid to isolated aleurone layers of barley (Hordeum vulgare L.) causes the production and secretion of four alpha-amylases. Two of these are stable at pH 3.7 and are not inactivated by ethylenediaminetetraacetate. The other two represent the classical barley alpha-amylases; i.e., they are inactivated at pH 3.7 and by reagents which from complexes with divalent metal ...
متن کاملGibberellic acid-controlled synthesis of alpha-amylase in barley endosperm.
Because the endosperm half of a barley seed produces several hydrolytic enzymes in response to added gibberellins (1, 4, 5, 8), it is an attractive experimental system for a study of the mechanism of action of gibberellic acid. The only living cells in the half seed are those of the aleurone layers. These are an apparently homogeneous collection of respiring, nondividing cells which have the hi...
متن کاملGibberellic Acid-Enhanced Synthesis and Release of a-Amylase and Ribonuclease by Isolated Barley Aleurone Layers
Summnary. Gibberellic acid enhances the synthesis of a-amylase in isolated aleurone layers of barley-seeds (Hordeumn vulgare var. Himalaya). In the presence of 20 mm calcium chloride the amount of enzyme obtained from isolated aleurone layers is quantitatively comparable to that of the half-seeds used in earlier studies. After a lag period of 6 to 8 hours enzyme is produced at a linear rajte. G...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1966
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.41.8.1395